Developer guide for fraud prevention APIs up with the many ways crooks evade security software is a full time job for fraud detection teams. That’s why it’s important for developers to grok the latest methods so that they can build safeguards into the products they build and sell.

The best way to prevent fraud is by using an API (application programming interface) – software that allows for seamless transfer of data between sites and apps. This enables a third party to analyze user or payment information in real time for signs of fraud, and users who are classified as higher risk can be subjected to additional verification or screening without impacting the end customer experience.

Sift empowers ecommerce businesses to break free from fraud with its fraud detection API. It’s built on the ethos that users are innocent until proven guilty, which ensures well intending customers aren’t unnecessarily turned away through rejection or a complicated user experience. Its scalable pricing model also lets businesses scale their use of the API to fit their business needs.

When to Use Fraud Prevention APIs

The right time to use a fraud prevention API is when a user takes an important action, such as signing up for an account, making a purchase, logging in or updating their billing information. The API can then instantly run a complete fraud detection system over the user or payment information to assess risk, and return a risk score and reputation metrics. This enables you to block users, payments or transactions and take further action depending on the score returned.…

Best ABHD5 antibody for WB, ELISA and Immunohistochemistry

Mouse anti human ABHD5 antibody
ABHD5 is an enzyme that binds to lipid substrates and activates lipase activity in lipid droplets (LDs). Mutations in ABHD5 that prevent binding to lipids or inhibit lipase activation impair ABHD5 function, suggesting that lipid interaction is important for ABHD5’s functional activity. ABHD5 is highly expressed in cancer cells and tissues and has been associated with tumor progression, poor survival, and resistance to ICB therapy. This link bosterbio.com

To investigate whether ABHD5 influences stem cell functions, we generated doxycycline-inducible BA cell lines stably expressing either wild-type or mutated ABHD5. These cellular populations were monitored by mCherry fluorescence, indicative of ABHD5 expression, and compared to doxycycline-inducible control cells stably expressing mCherry, which did not express ABHD5.

Anti-ABHD5 Antibody: Key Features and Uses

In LNCaP cells, a high level of mCherry expression was observed in ABHD5-knockdown cells, whereas in ABHD5-knockout cells the number of mCherry-positive colonies was significantly reduced in Matrigel Boyden chamber assays (Fig. 3B). Consistent with the observation that ABHD5 knockdown induced an invasive phenotype in PCa cells, mCherry-fluorescence and Matrigel Boyden chamber assays both indicated that ABHD5 deficiency enhances cell migration and invasion.

Mutant characterization of ABHD5 revealed that R299, G328, and D334 are important for ABHD5 lipase activation. Molecular dynamics simulations indicated that the spatial proximity between Ne2 of R299 and Od2 of D334 (a distance in a range of 13.8 A) suggests that a salt bridge may maintain a conformation required for ATGL-dependent lipolysis. Consequently, a charge-preserving mutation D334E, which is conserved in all vertebrate ABHD5 homologs, failed to trigger ATGL-dependent lipolysis.…